منابع مشابه
Three decades of nanopore sequencing
Received 6 January 2015; accepted 5 November 2015; published online 6 May 2016; doi:10.1038/nbt.3423 and together with Sergey Bezrukov at the US National Institutes of Health (NIH), had established the conditions that were necessary to avoid spontaneous gating (pore closure) of the a-hemolysin channel3. This was important, because spontaneous gating would have hindered, or confused, observation...
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A single molecule of Deoxyribonucleic acid (DNA) is being sequenced by using an innovative concept of Nanopore. The orientation of nanopore define the channel of ions through the pore comes into contact with nucleotide, the permutation and the transition kinematics of each molecule of DNA. The divergence of electrical signal observed when the four chemical bases adenine (A), guanine (G), cytosi...
متن کاملThe evolution of nanopore sequencing
The "$1000 Genome" project has been drawing increasing attention since its launch a decade ago. Nanopore sequencing, the third-generation, is believed to be one of the most promising sequencing technologies to reach four gold standards set for the "$1000 Genome" while the second-generation sequencing technologies are bringing about a revolution in life sciences, particularly in genome sequencin...
متن کاملNanopore DNA sequencing with MspA.
Nanopore sequencing has the potential to become a direct, fast, and inexpensive DNA sequencing technology. The simplest form of nanopore DNA sequencing utilizes the hypothesis that individual nucleotides of single-stranded DNA passing through a nanopore will uniquely modulate an ionic current flowing through the pore, allowing the record of the current to yield the DNA sequence. We demonstrate ...
متن کاملError analysis of idealized nanopore sequencing.
This numerical study provides an error analysis of an idealized nanopore sequencing method in which ionic current measurements are used to sequence intact single-stranded DNA in the pore, while an enzyme controls DNA motion. Examples of systematic channel errors when more than one nucleotide affects the current amplitude are detailed, which if present will persist regardless of coverage. Absent...
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ژورنال
عنوان ژورنال: Nature Biotechnology
سال: 2016
ISSN: 1087-0156,1546-1696
DOI: 10.1038/nbt.3423